Behavioral Research Blog

How to mark zebrafish without compromising their behavior

Posted by G. Smit on Mar 20, 2014

They may have just found the answer to this at the University of Toronto, Mississauga (Canada). Cheung et al. tried out a method using subcutaneous injection with dyes.

Clipping fins and adding tags
There have been many advances in methods and techniques for experiments with zebrafish, but identifying individuals seems like a difficult problem to tackle. You can’t just tell from the pattern of their stripes like with zebras, so even the trained eye can’t tell them apart. Many researchers use markings – clipping the fins in a specific way. It’s a relatively easy method, but because these fins are mainly transparent, these markings are difficult to see. Moreover, clipping fins might interfere with the fish’s swimming abilities, which poses a new problem, especially for behavioral studies.

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Topics: The Observer XT, animal behavior research, social behavior research, video observation, zebrafish, markers

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